RESUMO
We report the circularized complete genome sequences, containing a circular chromosome and two circular plasmids, of strains SalSpp05 (4.9 Mbp) and SalSpp10 (4.8 Mbp), which were isolated from chicken carcass rinse water samples; the sequences were obtained by combining Oxford Nanopore Technologies long-read data and Illumina short-read data. Whole-genome alignments indicated that both strains belong to Salmonella enterica.
RESUMO
Analysis of feed supplements can highlight microbial diversity and the prevalence of antimicrobial resistance (AMR), allowing users to monitor the safety of their animals. The 16S amplicon and metagenomic data generated by nanopore sequencing revealed that Bacillus was the dominant prokaryote, and AMR genes were detected in the animal probiotic products.
RESUMO
The complete mitochondrial genome of cobia, Rachycentron canadum, was reanalyzed and revised. The genome is 18,008 bp in length, containing 13 protein-coding genes, 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and a control region or displacement loop (D-loop). The gene arrangement is identical to that observed in most vertebrates. Base composition on the heavy strand is 30.14% A, 25.22% C, 15.80% G and 28.84% T. The D-loop region exhibits an A + T rich pattern, containing short tandem repeats of TATATACATGG, TATATGCACAA and TATATGCACGG. The mitochondrial genome studied differs from the previously published genome in two segments; the control region to 12S and ND5 to tRNA(Glu). The 12S sequence also differs from those published in the databases. Phylogeny analyses revealed that the differences could be due to errors in sequence assembly and/or sample misidentification of the previous studies.
